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protein expression vectors  (New England Biolabs)


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    Structured Review

    New England Biolabs protein expression vectors
    Protein Expression Vectors, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptyb21+expression+vector/pTYB21+Vector/pm37147085-379-2-21
    Average 94 stars, based on 87 article reviews
    protein expression vectors - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The overproduction ofSynechocystissp. PCC 6803 heat-shock protein (Sll0170) protectsEscherichia coliagainst high-temperature stress
    Article Snippet: .. Next, the 1.9 kbp DNA fragment was cloned into the pTYB21 expression vector (New England Biolab, UK) digested with the same restriction enzyme. ..

    Expressing:

    Article Title: The overproduction ofSynechocystissp. PCC 6803 heat-shock protein (Sll0170) protectsEscherichia coliagainst high-temperature stress
    Article Snippet: .. Next, the 1.9 kbp DNA fragment was cloned into the pTYB21 expression vector (New England Biolab, UK) digested with the same restriction enzyme. ..

    Article Title: An Extremely Stable Interprotein Tetrahedral Hg(Cys) 4 Core Forms in the Zinc Hook Domain of Rad50 Protein at Physiological pH
    Article Snippet: .. Expression and purification of metal‐free Hk130 : The production of PF Hk130 relied on a previously established protocol using the pTYB21 expression vector (IMPACT Protein Purification System, NEB) and Escherichia coli BL21‐CodonPlus (DE3)‐RIL strain. ..

    Article Title: Metal Exchange in the Interprotein Zn II ‐Binding Site of the Rad50 Hook Domain: Structural Insights into Cd II ‐Induced DNA‐Repair Inhibition
    Article Snippet: .. The production of P. furiosus Hk130 and human metallothionein‐2 (MT2) relied on a previously established protocol using the pTYB21 expression vector (IMPACT Protein Purification System, NEB) and Escherichia coli ( E. coli ) BL21‐CodonPlus (DE3)‐RIL strain. .. The expression vector is deposited in the Addgene plasmid repository ( https://www.addgene.org ), plasmid ID 105693 (MT2a).

    Article Title: Characterization of a glutamate-cysteine ligase in Bombyx mori.
    Article Snippet: The pGEM-T Easy Vector harboring the bmgcl insert was digested with the restriction enzymes NcoI and BamHI. .. The fragment obtained was subcloned into pTYB21 expression vector (New England Biolabs, Ipswich, MA, USA) for the expression of recombinant bmGCL. .. E. coli BL21 (DE3) pLysS cells (Promega, Madison, WI, USA) were transformed with this recombinant plasmid and incubated at 37 °C in Luria-Broth (LB) medium supplemented with 100 μg/mL ampicillin until the optical density (OD600nm) reached 0.5.

    Plasmid Preparation:

    Article Title: The overproduction ofSynechocystissp. PCC 6803 heat-shock protein (Sll0170) protectsEscherichia coliagainst high-temperature stress
    Article Snippet: .. Next, the 1.9 kbp DNA fragment was cloned into the pTYB21 expression vector (New England Biolab, UK) digested with the same restriction enzyme. ..

    other:

    Article Title: An Extremely Stable Interprotein Tetrahedral Hg(Cys) 4 Core Forms in the Zinc Hook Domain of Rad50 Protein at Physiological pH.
    Article Snippet: In nature, thiolate-based systems are the primary targets of divalent mercury (Hg) toxicity.. The formation of Hg(Cys)x cores in catalytic and structural protein centers mediates mercury’s toxic effects and ultimately leads to cellular damage.. Multiple studies have revealed distinct Hgthiolate coordination preferences, among which linear Hg complexes are the most commonly observed in solution at physiological pH.

    Purification:

    Article Title: An Extremely Stable Interprotein Tetrahedral Hg(Cys) 4 Core Forms in the Zinc Hook Domain of Rad50 Protein at Physiological pH
    Article Snippet: .. Expression and purification of metal‐free Hk130 : The production of PF Hk130 relied on a previously established protocol using the pTYB21 expression vector (IMPACT Protein Purification System, NEB) and Escherichia coli BL21‐CodonPlus (DE3)‐RIL strain. ..

    Protein Purification:

    Article Title: An Extremely Stable Interprotein Tetrahedral Hg(Cys) 4 Core Forms in the Zinc Hook Domain of Rad50 Protein at Physiological pH
    Article Snippet: .. Expression and purification of metal‐free Hk130 : The production of PF Hk130 relied on a previously established protocol using the pTYB21 expression vector (IMPACT Protein Purification System, NEB) and Escherichia coli BL21‐CodonPlus (DE3)‐RIL strain. ..

    Article Title: Metal Exchange in the Interprotein Zn II ‐Binding Site of the Rad50 Hook Domain: Structural Insights into Cd II ‐Induced DNA‐Repair Inhibition
    Article Snippet: .. The production of P. furiosus Hk130 and human metallothionein‐2 (MT2) relied on a previously established protocol using the pTYB21 expression vector (IMPACT Protein Purification System, NEB) and Escherichia coli ( E. coli ) BL21‐CodonPlus (DE3)‐RIL strain. .. The expression vector is deposited in the Addgene plasmid repository ( https://www.addgene.org ), plasmid ID 105693 (MT2a).

    Recombinant:

    Article Title: Characterization of a glutamate-cysteine ligase in Bombyx mori.
    Article Snippet: The pGEM-T Easy Vector harboring the bmgcl insert was digested with the restriction enzymes NcoI and BamHI. .. The fragment obtained was subcloned into pTYB21 expression vector (New England Biolabs, Ipswich, MA, USA) for the expression of recombinant bmGCL. .. E. coli BL21 (DE3) pLysS cells (Promega, Madison, WI, USA) were transformed with this recombinant plasmid and incubated at 37 °C in Luria-Broth (LB) medium supplemented with 100 μg/mL ampicillin until the optical density (OD600nm) reached 0.5.



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